Humphreys was in Europe when the war began, studying the natural sciences in Oxford, Madrid, and Berlin, where he earned a Ph.D. at the University of Berlin prior to returning to the C.S.A. Humphreys enlisted as a private in Co. C of the 12th N.C. Infantry in March, 1864. He was wounded in the face during the Battle of Spotsylvania Courthouse on May 19, 1864, fracturing his some of his facial bones. He was discharged due to disability on February 14, 1865 as his wound left him "unable to eat camp rations." He was paroled on April 15, 1865 in Lynchburg, VA.
After the war, he was acknowledged as an expert in the study of lepidoptera (moths and butterflies) and also published geological studies of North Carolina. He died in 1882 due in part to the effects of his war wounds.
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